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Fraction of bound B4F eluted from (a) M88 over 0.5 h, (b) M88 over 16 h, (c) WT over 0.5 h, and (d) WT over 16 h. All M88 samples are in the reduced form except one in panel A, indicated by (Ox). Elution was measured from streptavidin in solution, as well as conjugated to various <t>magnetic</t> <t>beads:</t> Dynabeads M‐270 (2.8 μm <t>diameter),</t> Dynabeads MyOne Streptavidin T1 (1 μm), Lunabeads (0.5 μm). ±1°C of random horizontal displacement was added to the data for clarity. Colored numbers indicate T 87.5 , the temperature at which 87.5% ligand release was achieved.
Carboxyl Coated Magnetic Beads Lunabeads, supplied by Luna Nanotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fraction of bound B4F eluted from (a) M88 over 0.5 h, (b) M88 over 16 h, (c) WT over 0.5 h, and (d) WT over 16 h. All M88 samples are in the reduced form except one in panel A, indicated by (Ox). Elution was measured from streptavidin in solution, as well as conjugated to various <t>magnetic</t> <t>beads:</t> Dynabeads M‐270 (2.8 μm <t>diameter),</t> Dynabeads MyOne Streptavidin T1 (1 μm), Lunabeads (0.5 μm). ±1°C of random horizontal displacement was added to the data for clarity. Colored numbers indicate T 87.5 , the temperature at which 87.5% ligand release was achieved.
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Fraction of bound B4F eluted from (a) M88 over 0.5 h, (b) M88 over 16 h, (c) WT over 0.5 h, and (d) WT over 16 h. All M88 samples are in the reduced form except one in panel A, indicated by (Ox). Elution was measured from streptavidin in solution, as well as conjugated to various <t>magnetic</t> <t>beads:</t> Dynabeads M‐270 (2.8 μm <t>diameter),</t> Dynabeads MyOne Streptavidin T1 (1 μm), Lunabeads (0.5 μm). ±1°C of random horizontal displacement was added to the data for clarity. Colored numbers indicate T 87.5 , the temperature at which 87.5% ligand release was achieved.
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Fraction of bound B4F eluted from (a) M88 over 0.5 h, (b) M88 over 16 h, (c) WT over 0.5 h, and (d) WT over 16 h. All M88 samples are in the reduced form except one in panel A, indicated by (Ox). Elution was measured from streptavidin in solution, as well as conjugated to various <t>magnetic</t> <t>beads:</t> Dynabeads M‐270 (2.8 μm <t>diameter),</t> Dynabeads MyOne Streptavidin T1 (1 μm), Lunabeads (0.5 μm). ±1°C of random horizontal displacement was added to the data for clarity. Colored numbers indicate T 87.5 , the temperature at which 87.5% ligand release was achieved.
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Fraction of bound B4F eluted from (a) M88 over 0.5 h, (b) M88 over 16 h, (c) WT over 0.5 h, and (d) WT over 16 h. All M88 samples are in the reduced form except one in panel A, indicated by (Ox). Elution was measured from streptavidin in solution, as well as conjugated to various <t>magnetic</t> <t>beads:</t> Dynabeads M‐270 (2.8 μm <t>diameter),</t> Dynabeads MyOne Streptavidin T1 (1 μm), Lunabeads (0.5 μm). ±1°C of random horizontal displacement was added to the data for clarity. Colored numbers indicate T 87.5 , the temperature at which 87.5% ligand release was achieved.
Hydrophilic And Hydrophobic Carboxylate–Coated Magnetic Beads Cytiva #65152105050250, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fraction of bound B4F eluted from (a) M88 over 0.5 h, (b) M88 over 16 h, (c) WT over 0.5 h, and (d) WT over 16 h. All M88 samples are in the reduced form except one in panel A, indicated by (Ox). Elution was measured from streptavidin in solution, as well as conjugated to various magnetic beads: Dynabeads M‐270 (2.8 μm diameter), Dynabeads MyOne Streptavidin T1 (1 μm), Lunabeads (0.5 μm). ±1°C of random horizontal displacement was added to the data for clarity. Colored numbers indicate T 87.5 , the temperature at which 87.5% ligand release was achieved.

Journal: Protein Science : A Publication of the Protein Society

Article Title: An engineered redox‐switchable streptavidin mutein enables the high‐affinity capture and efficient elution of biotinylated ligands

doi: 10.1002/pro.70224

Figure Lengend Snippet: Fraction of bound B4F eluted from (a) M88 over 0.5 h, (b) M88 over 16 h, (c) WT over 0.5 h, and (d) WT over 16 h. All M88 samples are in the reduced form except one in panel A, indicated by (Ox). Elution was measured from streptavidin in solution, as well as conjugated to various magnetic beads: Dynabeads M‐270 (2.8 μm diameter), Dynabeads MyOne Streptavidin T1 (1 μm), Lunabeads (0.5 μm). ±1°C of random horizontal displacement was added to the data for clarity. Colored numbers indicate T 87.5 , the temperature at which 87.5% ligand release was achieved.

Article Snippet: Dynabeads smaller than 1 μm are not available, but the more rapid elution from 0.5 μm diameter Carboxyl Coated Magnetic Beads (Luna Nanotech, herein referred to as Lunabeads) follows the same trend: T 87.5 is correlated with the size of the magnetic bead to which streptavidin was coupled, with smaller beads allowing elution at lower temperatures.

Techniques: Magnetic Beads